Welcome to iSTOP!

A database of sgRNAs for generating STOP codons (sgSTOPs) using CRISPR-dependent base editing

About iSTOP

Please see CRISPR-mediated base editing enables efficient disruption of eukaryotic genes through induction of STOP codons (iSTOP)", Billon P., Bryant E. et al. Molecular Cell, 2017

Software

iSTOP R Package-Design iSTOP guides for any annotated genome

iSTOP paper-Reproduce figures from Billon P., Bryant E. et al. Molecular Cell, 2017

Search for genes

Search for sgSTOPs to model nonsense mutations identified in cancer



Search for sgSTOPs to model cancer-associated nonsense mutations in gene(s) of interest





Filter the Search

Search for sgSTOPs to model nonsense mutations in cancer type(s) of interest

Do not select all of the cancer types







Filter the Search

Download the entire dataset for a species of interest

Click on the links to download the entire dataset for each species

Complete iSTOP Datasets

H. sapiens Dataset

M. musculus Dataset

R. norvegicus Dataset

D. rerio Dataset

C. elegans Dataset

D. melanogaster Dataset

A. thaliana Dataset

S. cerevisiae Dataset